Cell Meter Annexin V凋亡檢測試劑盒是美國AAT Bioquest生產(chǎn)的細胞凋亡檢測試劑盒，我們的Cell Meter 檢測試劑盒是一套用于檢測細胞生存力的工具。
數(shù)據(jù)分析
在活的非凋亡細胞中，膜聯(lián)蛋白V-iFluor 488檢測非誘導細胞中的先天性細胞凋亡，其通常為所有細胞的2-6％。 在凋亡細胞中，膜聯(lián)蛋白V-iFluor 488與磷脂酰絲氨酸結合，磷脂酰絲氨酸位于細胞膜的外部小葉上，因此導致染色強度增加。
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圖1.檢測膜聯(lián)蛋白V-iFluor 488和磷脂酰絲氨酸在Jurkat細胞中的結合活性。 Jurkat細胞在37℃，5％CO2培養(yǎng)箱中不用（藍色）或用20μM喜樹堿（紅色）處理4-5小時，然后用Annexin V-iFluor 488染色30分鐘。 使用FL1通道用FACSCalibur（Becton Dickinson）流式細胞儀測量膜聯(lián)蛋白V-iFluor 488的熒光強度。
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公司簡介
西安百螢生物科技有限公司是一家專注從事生物、化學試劑及其試劑盒的高新技術企業(yè)；主要以熒光探針，分子探針，核酸/蛋白質標記、細胞檢測等技術和產(chǎn)品為研發(fā)核心，產(chǎn)品應用于基因合成、細胞檢測、活體示蹤，抗體標記、新藥篩選等領域。